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. 2013 Jul 13;288(34):24581–24589. doi: 10.1074/jbc.M113.467506

FIGURE 3.

FIGURE 3.

Gel shift assay for E2F DNA-binding properties. A, probes used for gel shift assay. Probe Wt has two E2F sites derived from E2F1 promoter; Probe Mutant has the distal E2F site mutated. B, binding reactions contained the indicated probe along with cell lysate from 293 cells transfected with overexpression vectors encoding E2F1-(HA-DP1), Myc-E2F7, and E2F1-(HA-DP1)-(Myc-E2F7), respectively. Various antibodies were added to the reactions for distinguishing different protein-DNA complexes as indicated.