Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jul 13;288(34):24581–24589. doi: 10.1074/jbc.M113.467506

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — Gel shift assay for E2F DNA-binding properties. A, probes used for gel shift assay. Probe Wt has two E2F sites derived from E2F1 promoter; Probe Mutant has the distal E2F site mutated. B, binding reactions contained the indicated probe along with cell lysate from 293 cells transfected with overexpression vectors encoding E2F1-(HA-DP1), Myc-E2F7, and E2F1-(HA-DP1)-(Myc-E2F7), respectively. Various antibodies were added to the reactions for distinguishing different protein-DNA complexes as indicated.