FIGURE 1.

ADP^R of wild-type eEF2 in the presence of the dominant DT^R eEF2 mutant, I698A. A, strain TKY1367 containing the Gal-inducible DT plasmid (pLMY101), eEF2-His₆ (pTKB1051), and eEF2-I698A-HA (pTKB1050) was grown for 24 h in C-Ura medium with either 2% glucose or 2% galactose. Whole cell extracts were prepared, separated by SDS-PAGE, and immunoblotted with the indicated antibodies. B, tandem MS spectra of the ADP^R tryptic fragment ⁶⁸⁶VNILDVTLHADAIHR⁷⁰⁰ from eEF2-His₆ purified from the strain described in A. y ions are marked. Peaks that are marked with an asterisk are peaks of neutral loss of 601 Da.