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DT expression results in defects in cell separation. A, strain DBY12100 containing DTIND (pTKB1122) was grown in the presence or absence of β-estradiol for 24 h in C-Ura medium at 30 °C. Cells were then fixed, stained with DAPI, and imaged by confocal microscopy. Left panels, brightfield; right panels, DAPI. B, strain DBY12100 was grown as in A and fixed for EM. The right panels show a magnified view of the budneck. Arrowheads indicate the primary septa.
