TABLE 1.
S. cerevisiae strains and plasmids
| S. cerevisiae | Genotype | Ref. |
|---|---|---|
| Strain | ||
| W303 | Matα, leu2–3,112 trp1–1 can1–100 ura3–1 ade2–1 his3–11,15 | 41 |
| DBY12100 | Matα, (PGAL10+gal1)Δ::loxP, leu2Δ0::PACT1-GEV-NatMX, gal4Δ::LEU2, ura3Δ::HphMX4, HAP1+ | 30 |
| TKY1713 | Matα, (PGAL10+gal1)Δ::loxP, leu2Δ0::PACT1-GEV-NatMX, gal4Δ::LEU2, ura3Δ::HphMX4, dph2Δ::KanMX, HAP1+ | This study |
| TKY675 | MATa ade2 leu2 ura3 his3 leu2 trp1 eft1::HIS3 | 25 |
| eft2::TRP1 pEFT2-His6 LEU2 CEN | ||
| TKY825 | MATa ade2 leu2 ura3 his3 leu2 trp1 eft1::HIS3 | 4 |
| eft2::TRP1 pEFT2-His6 D696A LEU2 CEN | ||
| TKY1367 | MATa ade2 leu2 ura3 his3 leu2 trp1 eft1::HIS3 | This study |
| eft2::ADE2, pEFT2-His6 TRP1 CEN, pEFT2-HA I698A LEU2 CEN | ||
| pGAL1-DT URA3 2μ | ||
| Plasmid | ||
| pLMY101 | pGAL1-DT URA3 2μ | 24 |
| pTKB1122 | pGAL1-DT URA3 CEN | This study |
| pTKB1050 | pEFT2-HA I698A LEU2 CEN | This study |
| pTKB1051 | pEFT2-His6 TRP1 CEN | This study |