FIGURE 5.
Influence of GHS-R1a dimerization on Gq-induced GHS-R1a conformational changes. A, fluorescence emission spectra of bimane attached to Cys-304 in GHS-R1a in the absence of ligand. B, changes in maximum emission intensity of bimane attached to GHS-R1a in the absence and presence of Gαqβ1γ2 (receptor-to-G protein ratio, 1:10). 1a, GHS-R1a monomer; 1a:1a, GHS-R1a-GHS-R1a homodimer; 1a:1b, GHS-R1a-GHS-R1b dimer; 1a:BLT1, GHS-R1a:BLT1 “dimer.” The asterisk indicates which protomer is labeled with bimane. Data represent the mean ± S.D. from three independent experiments.