FIGURE 5.

14-3-3 proteins do not modulate the kinetics of homomeric GluK2b receptors in a heterologous system. A, in outside-out configuration, no detectable current was evoked by glutamate (1 mm, 100 ms) from HEK293 cells expressing GluK2b subunits. Shown is a representative trace of 10 independent experiments. B, representative whole-cell current traces from HEK293 cells transfected with GluK2b, together with either corresponding vector (dark trace) or 14-3-3τ (red trace). Cotransfection of 14-3-3τ (red trace) has no effect on the desensitization of homomeric GluK2b receptors. Right panel shows the summary data for control (n = 7) and 14-3-3τ (n = 10) group, respectively.