FIGURE 1.

Both mRNA and protein expressions of SH3BP5 are induced by Humanin. A, real-time PCR analysis of SH3BP5 and GAPDH mRNAs, prepared from mouse PHNs and human SH-SY5Y cells, incubated with 10 μm HN or distilled water (DW) for 24 h. This experiment was performed with n = 3; ***, p < 0.001; *, p < 0.05. B, F11 cells, transfected with the pCMV-human SH3BP5 vector, were immunostained with the SH3BP5 antibody (red), not preabsorbed (left), preabsorbed with GST (middle), or preabsorbed with GST-SH3BP5 (right), which had been immobilized onto glutathione-Sepharose, for 24 h. Nuclei were stained with DAPI (blue). C, immunocytochemical analysis of SH-SY5Y cells with the SH3BP5 antibody (red), incubated with 100 nm Humanin G (HNG) or distilled water (DW) in the presence or the absence of 5 μm AG490 for 24 h. Nuclei were stained with DAPI (blue).