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. 2013 Jul 16;288(34):24691–24704. doi: 10.1074/jbc.M113.469692

FIGURE 2.

FIGURE 2.

SH3BP5 is ubiquitously expressed and localized in mitochondria. A, immunohistochemical analysis of SH3BP5 expression in various mouse neuronal tissues using the SH3BP5 antibody (green). The SH3BP5 antibody was preincubated (preabsorbed) with GST (a negative control) or GST-SH3BP5 (immunizing protein), bound to glutathione-Sepharose, for 24 h. Nuclei were stained with DAPI (blue). B, F11 cells were immunostained with the SH3BP5 antibody (green) and the MitoTracker® (red). Nuclei were stained with DAPI (blue). The SH3BP5 antibody was preincubated (preabsorbed) with GST (a negative control) or GST-SH3BP5 (immunizing protein), bound to glutathione-Sepharose, for 24 h. Nuclei were stained with DAPI (blue).