FIGURE 1.

Growth curves (A) and lipid analysis (B) of complemented P. aeruginosa mutant strains. A, growth curves of P. aeruginosa PAO1 wild type and PAO1 ΔPA0919 deletion mutant strains, cultivated in AB medium (pH 5.3), were plotted against time. S.D. values (error bars) are indicated. B, P. aeruginosa variants were cultivated in AB medium (pH 5.3) for 24 h. After harvesting, polar lipids were extracted, analyzed by two-dimensional TLC, and quantified using Gelscan version 6.0 after molybdatophosphoric acid staining. All A-PG levels were related to the wild type strain. The specific position of A-PG is indicated by an arrow. PE, phosphatidylethanolamine.