TABLE 1.
Frequency of protein-induced microdomains, interconnection sites, and ILVs on GUVs
n is the number of vesicles analyzed in at least three independent experiments, and TF-chol corresponds to a GLM model where TopFluorPI(4,5)P2 is exchanged for TopFluor-cholesterol. In the GLM II model, C18:1 ß-d-glucosyl-ceramide is added, and TopFluorCeramide is used as label.
| Protein derivative | GUVs containing protein-induced microdomains | GUVs containing protein-induced interconnection sites | GUVs containing ILVs (with uptake of outside medium) |
|---|---|---|---|
| % | % | % | |
| AnxA2 (n = 152, GLM) | 25 | 9 | 9 |
| AnxA2 (n = 44, TF-chol) | 20 | 5 | 7 |
| A2t (n = 142, GLM) | 29 | 8 | 12 |
| A2t (n = 171, GLM II) | 15 | 9 | 5 |
| A2t (n = 43, TF-chol) | 19 | 9 | 5 |
| AnxA2 core (n = 134, GLM) | 21 | 4 | 5 |
| GUVs w/o protein (n = 215, GLM, TF-chol) | 0 | 0 | <1 |
| GUVs w/o protein (n = 134, GLM II) | 0 | 0 | <1 |