FIGURE 4.

MDC inhibits Mtbhsp60-mediated induction of IL-10 in THP-1 macrophages. A, PMA-differentiated THP-1 macrophages in a chamber slide were preincubated for 30 min without or with MDC (100 μm) followed by incubation with 10 μg/ml of Mtbhsp60-FITC at 37 °C for 15 min. Cells were fixed and washed, and the cell bound fluorescence was analyzed by confocal laser scanning microscopy. B, PMA-differentiated THP-1 macrophages were either left untreated or pretreated with 50 or 100 μm MDC and subsequently incubated with 3 μg/ml of Mtbhsp60. IL-10 cytokine level was estimated by EIA in various culture supernatants harvested after 48 h of incubation. Results shown are representative of three different experiments.