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. 2001 Jul 10;98(15):8720–8725. doi: 10.1073/pnas.151240898

Figure 3.

Figure 3

Histone deacetylase components required for transcriptional repression by pRB. (A) pRB-mediated repression in strains lacking deacetylase components. Either Gal4DB- or DB-pRB-expressing ARS-CEN vectors were transformed into strains deleted for components important for RPD3-dependent histone deacetylase; repression was determined by growth on SD, −His, 10 mM 3-AT plates after 5 days. White lines through plates are drawn for convenience. For each image, eight strains were from the same plate and were photographed at the same time. (B) MSI1/CAC3, but not CAC2, is required for repression by DB-pRB. Plates were treated exactly as described in A. (C) DB-pRB protein levels are unaltered in the Δrpd3 and Δsin3 strain backgrounds. Extracts loaded in each lane were equilibrated for total protein concentration. (D) A Δrpd3 strain was transformed with either DB or DB-pRB. Representative transformants were then transformed with vector, wild-type RPD3, or mutant alleles of RPD3 that specifically lack deacetylase activity. The SD, −His, 10 mM 3-AT plate was photographed after 5 days growth.