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PRMT6-R35A is catalytically active. A PRMT6 cell-free methylation assay was performed with HIV-1 Rev protein, a known substrate of PRMT6. The upper frame represents the autoradiograph of Rev methylation by PRMT6. The lower frame represents a Coomassie stained gel of Rev. This experiment was performed three times with similar results obtained each time; a representative result is shown.
