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. 2013 Jun 5;122(8):1341–1349. doi: 10.1182/blood-2013-03-478255

Figure 6.

Figure 6

ZFN-mediated elimination of HLA-A expression on human ESC. The HLA-A2+HLA-24+hES parental cell line WIBR3 was modified by ZFN and donor plasmid coding for antibiotic resistance. Clones (5230, 5255, 5258) were chosen with loss of HLA-A expression and differentiated into fibroblasts. Expression of HLA-A2 and HLA-A24 on derived fibroblasts was assessed by flow cytometry after culturing with 600 IU/mL IFN-γ and 10 ng/mL TNF-α for 48 hours. Dashed line in parental panel represents isotype control.

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