Table 1.
Primers used for pyrosequencing and/or qPCR.
| Primer | Sequence (5′ – 3′)a | Target | Ta (°C) | References |
|---|---|---|---|---|
| Mlas | GGTGGTGTMGGDTTCACMCARTA | mcrA gene | 55 | Steinberg and Regan, 2008 |
| mcrA-rev | CGTTCATBGCGTAGTTVGGRTAGT | |||
| DSRp2060F | CAACATCGTYCAYACCCAGGG | dsrB gene | 53 | Dar et al., 2006; Geets et al., 2006 |
| Dsr-4Rdeg | GTGTARCAGTTDCCRCA | |||
| 27f | AGAGTTGATYMTGGCTCAG | Bacterial 16S rDNA (for pyrosequencing) | 52 | Giovannoni et al., 1988 |
| 536r | GTATTACCGCGGCKGCTG | |||
| 338f | ACTCCTACGGGAGGCAGC | Bacterial 16S rDNA (for qPCR) | 52 | Giovannoni, 1991 |
| 536r | GTATTACCGCGGCKGCTG | |||
| Arch340F | CCCTAYGGGGYGCASCAG | Archaeal 16S rDNA | 57 | Gantner et al., 2011 |
| Arch1000R | GAGARGWRGTGCATGGCC |
a
M, A/C; D, A/G/T; R, A/G; B, C/G/T; V, A/C/G; Y, C/T; K, G/T; S, G/C; W, A/T.