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. 2013 Aug 9;11:91. doi: 10.1186/1741-7007-11-91

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Copyright © 2013 Manov et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of Spalax, mouse and rat conditioned media on morphology and cell cycle progression in HepG2 cells. HepG2 cells were incubated under conditioned media for eight days; thereafter, cell morphology was documented using phase contrast microscopy, harvested, stained with PI and analyzed by flow cytometry. Representative images (×200) and flow cytometry histograms are presented: (A) control media; (B) rat CM; (C) mouse CM; (D) Spalax CM; (E) BrdU incorporation assay: HepG2 were grown in 96-well plates (2000 cells/well) for four and seven days under Spalax-generated CM. BrdU Cell Proliferation ELISA (Exalpha) was used. Time-dependent decrease in cell proliferation under Spalax-generated CM is depicted. CM, Conditioned media; PI, Propidium iodide.