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. 2001 Jul 10;98(15):8732–8737. doi: 10.1073/pnas.161126098

Figure 6.

Figure 6

Restoration of homeostatic proliferation of OT-I cells in IL-7− mice by addition of exogenous IL-7 but not by up-regulation of bcl-2. (Left) Groups of irradiated B6.IL-7− mice were injected with RAG-1− OT-I LN cells. For in vivo analysis, mice were either injected with PBS or 500 ng of recombinant IL-7 every 12 h for 7 days. For in vitro analysis, spleen fragments from the hosts were treated with media only or media containing 40 ng/ml IL-7 for 7 days. Irradiated B6 hosts injected with OT-I cells and analyzed either in vivo or in vitro conditions served as controls. Shown are gated donor cells. (Right) A group of irradiated B6 mice was injected with a mixture of wild-type B6.PL and B6.Ly5.1 bcl-2 transgenic cells. Mice were analyzed 7 days later by staining LN cells for Thy-1.1, Ly5.1, and CD8; shown are gated CD8+ cells.