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. 2013 Aug 6;13:111. doi: 10.1186/1471-2229-13-111

Figure 1.

Figure 1

Schematic showing the addition of the barcode tag during PCR amplification. In the initial cycles, M13(−21) tails are incorporated into the PCR product however due to the limited amount of specific M13(−21) primer this is replaced with the labelling with the barcode in subsequent cycles.