Table 2.
Numerical breakdown of read distribution on the three Illumina lanes for BAT mutation detection in all FAD2 orthologues and BnaC.GL2.b
| Reads | 4 | 5 | 6 | Average |
|---|---|---|---|---|
|
Total |
62,071,296 |
38,839,694 |
69,785,822 |
56,898,937 |
|
Spike |
3,128,126 |
1,554,316 |
3,693,148 |
2,791,863 |
|
Unmatched |
23,262,622 |
12,912,128 |
22,169,416 |
19,448,055 |
|
M13 detected |
17,840,274 |
12,186,625 |
21,961,629 |
17,329,509 |
|
6 bp BAT |
15,859,982 |
11,258,110 |
19,398,277 |
15,505,456 |
|
5 bp BAT |
591,667 |
338,953 |
984,976 |
638,532 |
|
4 bp BAT |
304,755 |
127,279 |
440,769 |
290,934 |
|
<4 bp BAT |
788,808 |
302,764 |
891,331 |
660,968 |
|
Munged |
32,925,286 |
23,213,290 |
40,815,100 |
32,317,892 |
|
Mapped BnaA.FAD2.a |
1,566,912 |
1,420,311 |
2,143,813 |
1,710,345 |
|
Mapped BnaA.FAD2.b |
6,579,001 |
4,804,826 |
8,002,197 |
6,462,008 |
|
Mapped BnaC.FAD2.a |
791,572 |
644,265 |
1,026,912 |
820,916 |
| Mapped BnaC.GL2.b | 12,302,580 | 6,167,201 | 15,136,272 | 11,202,018 |