Table 3.
List of mutations detected within the three Brassica orthologues of FAD2 and BnaC.GL2.b
| Lane | BAT | Gene | Amplicon position | Base |
Reads |
Ratio | Wild type AA | Mutant AA | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | C | G | T | |||||||||
| 6 |
B00146 |
BnaC.FAD2.a |
37 |
G |
183 |
0 |
1640 |
0 |
0.111585 |
Glu |
Lys |
|
| 5 |
B00282 |
BnaC.FAD2.a |
45 |
C |
0 |
3555 |
0 |
246 |
0.069198 |
Leu |
Leu |
|
| 4 |
B00009 |
BnaC.FAD2.a |
46 |
C |
7 |
5431 |
2 |
77 |
0.014178 |
Glu |
STOP |
|
| 4 |
B00282 |
BnaC.FAD2.a |
57 |
C |
0 |
2032 |
1 |
164 |
0.080709 |
Ile |
Ile |
|
| 4 |
B00136 |
BnaC.FAD2.a |
59 |
C |
0 |
2176 |
0 |
59 |
0.027114 |
Ser |
Phe |
|
| 4 |
B00077 |
BnaC.FAD2.a |
68 |
G |
137 |
0 |
1524 |
1 |
0.089895 |
Gly |
Asp |
|
| 6 |
B00087 |
BnaC.FAD2.a |
227 |
C |
0 |
143 |
0 |
14 |
0.097902 |
Ser |
Leu |
|
| 6 |
B00040 |
BnaC.FAD2.a |
249 |
G |
11 |
0 |
931 |
0 |
0.011815 |
Arg |
Arg |
|
| 6 |
B00207 |
BnaA.FAD2.a |
43 |
C |
3 |
14174 |
0 |
147 |
0.010371 |
N/A |
N/A |
|
| 4 |
B00108 |
BnaA.FAD2.a |
45 |
C |
2 |
12350 |
0 |
319 |
0.02583 |
N/A |
N/A |
|
| 5 |
B00136 |
BnaA.FAD2.a |
45 |
C |
0 |
4566 |
0 |
298 |
0.065265 |
N/A |
N/A |
|
| 6 |
B00041 |
BnaA.FAD2.a |
45 |
C |
1 |
5883 |
0 |
68 |
0.011559 |
N/A |
N/A |
|
| 4 |
B00208 |
BnaA.FAD2.a |
48 |
G |
309 |
0 |
5574 |
0 |
0.055436 |
N/A |
N/A |
|
| 5 |
B00119 |
BnaA.FAD2.a |
48 |
G |
940 |
8 |
15594 |
0 |
0.06028 |
N/A |
N/A |
|
| 6 |
B00114 |
BnaA.FAD2.a |
63 |
C |
1 |
8285 |
0 |
88 |
0.010622 |
N/A |
N/A |
|
| 6 |
B00147 |
BnaA.FAD2.a |
63 |
C |
2 |
9325 |
0 |
861 |
0.092332 |
N/A |
N/A |
|
| 4 |
B00108 |
BnaA.FAD2.a |
64 |
G |
1065 |
3 |
10533 |
0 |
0.101111 |
N/A |
N/A |
|
| 6 |
B00134 |
BnaA.FAD2.a |
69 |
C |
4 |
8271 |
0 |
237 |
0.028654 |
N/A |
N/A |
|
| 4 |
B00058 |
BnaA.FAD2.a |
225 |
C |
2 |
5339 |
0 |
316 |
0.059187 |
N/A |
N/A |
|
| 6 |
B00075 |
BnaA.FAD2.a |
238 |
G |
181 |
0 |
1314 |
1 |
0.137747 |
N/A |
N/A |
|
| 4 |
B00044 |
BnaA.FAD2.a |
248 |
G |
40 |
0 |
2640 |
0 |
0.015152 |
N/A |
N/A |
|
| 5 |
B00282 |
BnaA.FAD2.a |
248 |
G |
29 |
0 |
846 |
2 |
0.034279 |
N/A |
N/A |
|
| 4 |
B00073 |
BnaA.FAD2.b |
37 |
G |
53 |
0 |
1062 |
0 |
0.049906 |
N/A |
N/A |
|
| 5 |
B00285 |
BnaA.FAD2.b |
39 |
G |
27 |
0 |
1471 |
0 |
0.018355 |
N/A |
N/A |
|
| 5 |
B00213 |
BnaA.FAD2.b |
39 |
G |
6170 |
36 |
59711 |
4 |
0.103331 |
N/A |
N/A |
|
| 6 |
B00128 |
BnaA.FAD2.b |
40 |
C |
9 |
30818 |
0 |
1156 |
0.037511 |
N/A |
N/A |
|
| 4 |
B00027 |
BnaA.FAD2.b |
43 |
C |
26 |
72577 |
1 |
6652 |
0.091654 |
N/A |
N/A |
|
| 4 |
B00180 |
BnaA.FAD2.b |
45 |
C |
9 |
26921 |
0 |
824 |
0.030608 |
N/A |
N/A |
|
| 4 |
B00260 |
BnaA.FAD2.b |
45 |
C |
11 |
31793 |
2 |
1069 |
0.033624 |
N/A |
N/A |
|
| 5 |
B00135 |
BnaA.FAD2.b |
45 |
C |
1 |
4675 |
0 |
114 |
0.024385 |
N/A |
N/A |
|
| 5 |
B00211 |
BnaA.FAD2.b |
45 |
C |
9 |
67246 |
3 |
5206 |
0.077417 |
N/A |
N/A |
|
| 5 |
B00243 |
BnaA.FAD2.b |
45 |
C |
5 |
87630 |
1 |
4416 |
0.050394 |
N/A |
N/A |
|
| 6 |
B00299 |
BnaA.FAD2.b |
48 |
G |
335 |
1 |
13245 |
1 |
0.025293 |
N/A |
N/A |
|
| 4 |
B00090 |
BnaA.FAD2.b |
59 |
C |
34 |
127784 |
1 |
2404 |
0.018813 |
N/A |
N/A |
|
| 5 |
B00087 |
BnaA.FAD2.b |
59 |
C |
3 |
8544 |
0 |
744 |
0.087079 |
N/A |
N/A |
|
| 5 |
B00223 |
BnaA.FAD2.b |
63 |
C |
28 |
64330 |
0 |
12219 |
0.189942 |
N/A |
N/A |
|
| 6 |
B00250 |
BnaA.FAD2.b |
63 |
C |
2 |
1965 |
0 |
413 |
0.210178 |
N/A |
N/A |
|
| 5 |
B00111 |
BnaA.FAD2.b |
68 |
G |
249 |
1 |
3179 |
1 |
0.078327 |
N/A |
N/A |
|
| 6 |
B00195 |
BnaA.FAD2.b |
219 |
C |
0 |
2666 |
0 |
28 |
0.010503 |
N/A |
N/A |
|
| 6 |
B00118 |
BnaA.FAD2.b |
237 |
G |
20 |
0 |
2055 |
0 |
0.009732 |
N/A |
N/A |
|
| 5 |
B00105 |
BnaA.FAD2.b |
265 |
G |
97 |
0 |
352 |
0 |
0.275568 |
N/A |
N/A |
|
| 6 |
B00118 |
BnaC.GL2.b |
46 |
C |
14 |
46512 |
3 |
1058 |
0.022747 |
intron |
|
|
| 5 |
B00249 |
BnaC.GL2.b |
65 |
G |
330 |
2 |
20270 |
9 |
0.01628 |
Ile |
Ile |
|
| 4 |
B00104 |
BnaC.GL2.b |
68 |
C |
1 |
7275 |
1 |
67 |
0.00921 |
Arg |
Arg |
|
| 4 |
B00064 |
BnaC.GL2.b |
109 |
C |
6 |
32767 |
4 |
121 |
0.003693 |
Val |
Met |
|
| 4 |
B00307 |
BnaC.GL2.b |
110 |
C |
7 |
38693 |
2 |
784 |
0.020262 |
Ala |
Ala |
|
| 4 |
B00076 |
BnaC.GL2.b |
113 |
C |
5 |
32262 |
0 |
70 |
0.00217 |
Lys |
Lys |
|
| 6 |
B00091 |
BnaC.GL2.b |
122 |
C |
11 |
78003 |
2 |
1427 |
0.018294 |
Leu |
Leu |
|
| 6 |
B00228 |
BnaC.GL2.b |
130 |
C |
5 |
16523 |
2 |
191 |
0.01156 |
Ala |
Thr |
|
| 5 |
B00147 |
BnaC.GL2.b |
143 |
C |
1 |
14670 |
4 |
220 |
0.014997 |
Trp |
STOP |
|
| 6 | B00115 | BnaC.GL2.b | 143 | C | 17 | 76370 | 43 | 347 | 0.004544 | Trp | STOP | |