Figure 1:

Experiment time course. Mice in coinjection group were injected with mixture of human ovarian cancer cells and CV1 (control), CV1-FHC (ferritin), or CV1-FHC-FC (ferritin + FC) fibroblasts. Whole body in vivo fluorescence imaging (IVIS) was performed immediately after and 3 and 10 days after cell injection. MR imaging was performed at 3, 5, and 10 days after cell injection. For recruitment group, cancer cells were injected into mice, and on day 4, either CV1 or CV1-FHC-FC fibroblasts were injected intraperitoneally at site remote from tumor. Recruitment of CV1-FHC cells was examined with MR imaging immediately after and 2, 4, 7, and 9 days after remote injection. At 7 days after injection, IVIS imaging was used to confirm recruitment of CV1 cells. At conclusion of both protocols, tumors were removed for histologic examination.