Figure 1.
Comparative evaluation of PCR limit of detection from published malaria PCR assays. Data showing TaqMan probe assays performed in the background of QuantiFast Master Mix. Assays were performed in triplicate. Only Cq values of 40 and below were considered and only two out of three values were required to call the results a positive. LoD was established which contained the lowest parasite concentration expressed as parasites/mL.