Table 1.
“-omics” techniques for the investigation of plant HSR.
| “-omics” | Technique | Reference |
|---|---|---|
| Genomics | RAD (restriction site-associated DNA) | Pfender et al. (2011) |
| Whole-genome sequencing | Igarashi et al. (2013) | |
| Transcriptomics | RNA-seq | Wang et al. (2009) |
| Digital gene expression: MACE (massive analysis of cDNA ends) | Kahl et al. (2012) | |
| small RNA sequencing | Wei et al. (2011) | |
| Immunoprecipitation of sRNA-binding proteins | Windbichler et al. (2008) | |
| RNA– degradome sequencing | German et al. (2008) | |
| Epigenomics | ST-MSDK (methylation-specific digital karyotyping) | Hu et al. (2006) |
| Bisulphite sequencing direct | Frommer et al. (1992) | |
| Nearest neighbor TLC | Ramsahoye et al. (2000) | |
| MeDIP (methylated DNA immunoprecipitation) | Mohn et al. (2009) | |
| Anti-mC immunological techniques (5-methylcytosine antibody) | Sano et al. (1980) | |
| LC– mass spectrometry (LC/MS separation) | Annan etal. (1989) | |
| HPCE (capillary electrophoretic separation) | Fraga et al. (2002) | |
| Histone-modification detection (chromatin immunoprecipitation (ChIP) assay) | O’Neill and Turner (2003) | |
| Proteomics | Liquid chromatography/tandem mass spectrometry (LC–MS/MS) | Holmes-Davis et al. (2005) |
| Matrix-assisted laser desorption/ionization/time-of-flight mass spectrometry (MALDI-TOF(/TOF)MS) | Zou et al. (2009) | |
| MALDI-TOF MS, LC–MS/MS | Noir et al. (2005); Dai et al. (2006, 2007) | |
| Isotope-coded affinity tags (ICAT), LC–MS/MS | Grobei et al. (2009) | |
| Isobaric tags for relative and absolute quantification (ITRAQ), LC–MS/MS | Petersen et al. (2006) | |
| Gas chromatography –mass spectrometry (GC –MS) | Lisec et al. (2006) | |
| Shotgun proteomic approach (Gel-LC-Orbitrap-MS) | Chaturvedi et al. (2013) | |
| Metabolomics | Liquid chromatography (LC)–MS | Halket et al. (2005); Moco et al. (2007), Allwood and Goodacre (2010) |
| Capillary electrophoresis (CE)–MS | Monton and Soga (2007) | |
| Nuclear magnetic resonance (NMR) spectroscopy | Schripsema (2010) |