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. 2013 Aug 6;13:110. doi: 10.1186/1471-2229-13-110

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Copyright © 2013 Zhao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The expression of stress-responsive genes in transgenic GbRLK Arabidopsis vs. nontransgenic Col-0. Relative expression levels of stress-responsive genes were determined by qRT-PCR using cDNA synthesized from total RNAs isolated from the leaves of 2-week-old Arabidopsis grown in soil under normal conditions. Relative gene expression levels were determined using the 2^-ΔΔCT method. The CT (cycle threshold) values for both the target and internal control genes were means of three technical replicates. ΔCt = Ct target gene-CtRuBisCo. ΔΔCT = (CTtarget -CTRuBisCo)Transgenic - (CTtarget - CTRuBisCo) Non-transgenic. The large subunit of the RuBisCo gene (AtRuBisCo-F3/R3; Additional file 1: Table S1) from Arabidopsis was used as an internal control for normalization of different cDNA samples. The primers for the amplification of stress-responsive genes (Additional file 1: Table S1) were design based on sequences obtained from NCBI using Primer 5.0 software. Error bars represent standard error of means based on three independent reactions.