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. 2013 Aug 23;3:2498. doi: 10.1038/srep02498

Figure 3. Role of Pax3 in cardiac differentiation.

Figure 3

(A) Clones of P19 cells expressing either a dominant negative Meox1 (Meox1/EnR) or Pax3 (Pax3/EnR) were treated with bexarotene (BEX, 100 nM) or RA (10 nM) during EB formation. The cells were then maintained on coverslips without any treatments for an additional 5 days and stained with specific antibodies for MyoD, myosin heavy chain and Hoechst for the nuclei. Cells harbouring the empty vector were used as controls. Quantification is plotted as the fraction of cardiac myocytes (car, dark grey) and skeletal myocytes (sk, light grey) in relation to the total cell population (n = 5). (B)–(G) Real-time RT-PCR was used to determine the transcript levels of EnR, Meox1, Myf5, GATA4, Nkx2.5 and Tbx5 on day 4 differentiation in the same batch of cDNA from the cells harboring the dominant negative Pax3. Quantification is presented as fold changes in reference to the untreated EBs (n = 3).