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. 2013 Aug 6;12:89. doi: 10.1186/1476-4598-12-89

Figure 3.

Figure 3

Immunostaining for γ-H2AX and 53BP1 in Klf4+/+ and Klf4−/−MEFs. (A) Immunostaining was conducted for γ-H2AX and 53BP1 in Klf4+/+ and Klf4−/−MEFs. Non-irradiated Klf4+/+ and Klf4−/−MEFs were stained with antibodies against γ-H2AX and 53BP1 and hoechst stain (blue) was used to visualize nuclei. Shown is a representative result of three independent experiments. (B) Histogram showing quantification of cells with ≥ 5 γ-H2AX foci in Klf4+/+ and untransfected Klf4−/−MEFs with and without γ-irradiation. (C ) Histogram showing quantification of cells with ≥ 5 53BP1 foci in Klf4+/+ and untransfected Klf4−/−MEFs with and without γ-irradiation. For (B) and (C), foci were counted for non-irradiated cells, and at 1, 4 and 24h post irradiation. One hundred cells were counted per cell type per experiment. N = 5; * p < 0.05, ** p < 0.01 compared to Klf4+/+ cells.