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Determination of ploidy in Klf4+/+ and Klf4−/−MEFs. Karyotype analysis was conducted in metaphase chromosome spreads prepared from Klf4+/+ and from Klf4−/−MEFs transfected or not with GFP or Klf4-GFP. (A) Shown is a typical result of three independent experiments for Klf4+/+ and Klf4−/−MEFs. (B-E) Histograms showing quantification of chromosome number in Klf4+/+ and in Klf4−/−MEFs untransfected or transfected with GFP or Klf4-GFP. Spreads from 100 cells were examined per genotype.
