Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Aug 5;12:87. doi: 10.1186/1476-4598-12-87

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Korah et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Enforced expression of RASSF1A in ACC cells. (A) SW-13 ACC cells that do not express RASSF1A (a) were transfected with empty (a &e), RASSF1A(b - d) or RASSF1A/A133S (f - h) expression vectors. Expression of RASSF1A was determined by immunofluorescence detection of RASSF1A protein (b &f) or DDK tag (c &g) using anti-RASSF1 goat polyclonal and anti-DDK mAb respectively followed by anti-goat-FITC and anti-moue-TR secondary antibodies and DAPI for nuclear staining. Note co-localization of RASSF1A and DDK antigens (d &h) and absence of both in a &e. (B &C) Transient transfection was carried out in 6-well plates with a starting density of 80,000 cells/well and allowed to grow for 6 days to test the effect of RASSF1A and RASSF1A/A133S expression on growth potential (B) and survival (C) of SW-13 cells. Graphs represent one of 3 independent experiments that yielded similar results.