Table 1. Summary of spectra catalyzed by wild type and mutant Lcr luciferases.
| Luciferase | λmax (nm) at pH 8.0 (quantum yield) | λmax (nm) of the Gaussian components (ΦGC of each component at pH 8.0*) | ||
|---|---|---|---|---|
| Ppy WT | 558 (40.8%) | 560 (25.5%) | 605 (9.8%) | 649 (5.4%) |
| Lcr WT | 566 (37.2%) | 560 (22.6%) | 608 (9.9%) | 654 (5.2%) |
| Y257F | 598 (27.6%) | 570 (7.2%) | 610 (13.3%) | 650 (7.0%) |
| Y257A | 610 (18.6%) | 573 (0.9%) | 612 (12.0%) | 666 (5.9%) |
| Y257E | 608 (12.4%) | — (~0%) | 608 (7.7%) | 656 (4.5%) |
| Y257R | 594 (25.5%) | — (0%) | 594 (17.8%) | 650 (7.8%) |
*The values were calculated from the fitting data so that the summation of the three ΦGC deviated a little from the experimental value.