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Knockdown of ncc increases HR cell number and Na+ accumulation. (A, B): H+-ATPase (H-pump) immunostaining signals (arrow) in 4-dpf wild type larvae (WT) and ncc morphants. Scale bar = 100 μm. (C, D): Comparisons of HR cell density (C) and whole body Na+ content (D) between wild type larvae (white bars) and ncc morphants (gray bars) at different developmental stages (n = 8 in C and 6 in D). Mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test).
