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. 2013 Jul 16;44(1):53. doi: 10.1186/1297-9716-44-53

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Copyright © 2013 Ouyang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Viral load in gill and kidney. Common carp (n = 36 fish per tank), with an average weight of 10.7 g ± 3.2 g (mean ± SD), were inoculated by immersion for 2 h in water containing 100 PFU/mL of the indicated CyHV-3 strains. At different times post-inoculation, six infected fish were randomly selected per tank, euthanized and dissected. Six mock-infected fish were used as negative controls. Gill, kidney and spleen were harvested and stored in RNAlater® at −80 °C. DNA was extracted from gill (panel A) and kidney (panel B) and analyzed by real-time TaqMan PCR for quantification of viral genome copies. The results are expressed as the means ± SD of the data observed for the 6 fish analyzed per time point. Spleen were treated for quantification of carp gene expression by RT-qPCR (see Figure 11).