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. 2013 Jul 16;44(1):53. doi: 10.1186/1297-9716-44-53

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Copyright © 2013 Ouyang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Structural analysis of the FL BAC ORF134 recombinant plasmids and derived CyHV-3 recombinant strains. The CyHV-3 FL BAC, FL BAC ORF134 Del and FL BAC ORF134 Rev plasmids and the genome of the FL BAC revertant, FL BAC revertant ORF134 Del and FL BAC revertant ORF134 Rev strains were analyzed by SacI restriction (left panel) and further tested by southern blotting using probes corresponding to ORF55 (central panel) and ORF134 (right panel). White-outlined black arrowheads and white arrows indicate restriction fragments containing ORF55 and ORF134 loci, respectively. Marker sizes (MS) are indicated on the left.