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. Author manuscript; available in PMC: 2013 Aug 23.
Published in final edited form as: Mol Cell. 2011 Apr 8;42(1):50–61. doi: 10.1016/j.molcel.2011.03.017

Figure 1. Rac1 Deletion Reduced the Size of MEFs and Lymphocytes.

Figure 1

(A) Fl/+ and fl/fl MEFs were grown in 10% serum in the presence and absence of 4-HT for 48 hr (1: fl/+ treated with 4-HT, 2: fl/fl treated with ethanol, 3: fl/fl treated with 4-HT). Cells were lysed and subjected to SDS-PAGE and WB to assess Rac1 deletion.

(B) Rac1 fl/+ and fl/fl MEFs were grown in 10% serum, resuspended in Trypsin, diluted in PBS, then subjected to cell size analysis on a Beckman Coulter cell sorter before and after 4-HT treatment.

(C–F) B cells (C and D) and T cells (E and F) from WT, Rac1 fl/fl, Rac2−/− and Rac1 fl/fl Rac2−/− mice were grown in RPMI with 10 % serum for 48 hr in the presence of 500 nM 4-HT. Cells were resuspended in PBS, then subjected to FACscan analysis, based on forward scatter/side scatter (FS/SSC). The histograms in (D) and (F) resulted from the quantification of the cell size on the Forward Scatter scale. Error bars in (B), (D), and (F) specify the standard deviation between four independently performed experiments. Results are representative of four experiments.