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. Author manuscript; available in PMC: 2013 Aug 23.
Published in final edited form as: Mol Cell. 2011 Apr 8;42(1):50–61. doi: 10.1016/j.molcel.2011.03.017

Figure 2. Rac1 Regulates mTOR Activity in MEFs and HeLa Cells.

Figure 2

(A and B) WT (A) and Rac1 fl/fl (B) MEFs were cultured in DMEM with 10% serum. They were treated with ethanol (control) or with 500 nM 4-HT for 48 hr to delete Rac1. In some conditions, cells were treated for 1 hr with 100 nM wortmannin or 100 nM rapamycin at 37°C.

(C) Rac1 or control RNAi oligos were introduced into HeLa cells before analysis.

(D) Immortalized MEFs were infected or not with control or Rac1-expressing lentiviral particles, then incubated or not with 4-HT for 48 hr with serum-starvation for the last 14 hr. They were then stimulated or not for 10 min with 10% serum supplemented with 50 nM PDGF. The cells were then lysed and subjected to SDS-PAGE and western blot analysis. These results are representative of three experiments.