Skip to main content
. Author manuscript; available in PMC: 2013 Aug 23.
Published in final edited form as: Mol Cell. 2011 Apr 8;42(1):50–61. doi: 10.1016/j.molcel.2011.03.017

Figure 4. Rac1 Is Colocalized with mTOR and Is Required for Subcellular Localization of mTOR.

Figure 4

(A and B) MEFs were serum-starved (a) or serum starved then stimulated with 10% serum for 10 min (B). Cells were fixed and immunostained for Rac1 and mTOR and analyzed by immunofluorescence.

(C and D) MEFs were serum starved (C) or serum starved then stimulated with 10% serum, supplemented with 50 nM PDGF for 10 min (D). They were fixed and immunostained for Rac1 and mTOR and subjected to confocal microscopy analysis as described in the method section.

(E and F) Rac1 fl/fl MEFs were incubated with 4-HT for 2 days. They were serum starved (E) and serum-starved then stimulated for 10 min (F). Cells were fixed and stained for Rac1 and mTOR. Cells were visualized by immunofluorescence as described in the methods section. Images are representative of four experiments.