Figure 6.

Influence of the individual number of alleles (A), and the lowest read frequency (B) on the minimum number of reads required to determine a complete genotype with a 99.9% confidence level. Each point represents a different individual (N = 36). The minimum number of reads required was a posteriori computed (T1_Resampled) by resampling both amplicon replicates (T1_Resampled1, T1_Resampled2) and the maximal value was retained. The dashed line in (A) illustrates T1_{Galan Simul} computed according to Galan’s et al. [13] definition of an allele’s dropout (i.e. an allele associated with two reads or less; see text for details).