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View full-text article in PMC

. 2013 Jul 26;3:39. doi: 10.1186/2191-0855-3-39

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Copyright ©2013 Jakob et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Activity studies and SDS-PAGE visualization of produced CelA2, BSLA, subtilisin Carlsberg wild type. a) Activity measurements are performed with not optimized (left column) and mutagenized vector backbones (right column) in 96-well microtiter plate. Constructs with an M1 label habor an epMEGAWHOP optimized vector backbone; b) Visualization of production levels of CelA2 (proteins in supernatant of lysate), BSLA and subtilisin Carlsberg (proteins in culture supernatant after precipitation) by SDS-PAGE in combination with Coomassie brilliant blue staining. The reported relative activity values are the average of three 96-well microtiter plate measurements in which each construct was expressed 8 times per plate. Deviations are calculated from the corresponding mean values.