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. 2013 Jul 26;3:39. doi: 10.1186/2191-0855-3-39

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Copyright ©2013 Jakob et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Functional study of the lacI repressor under induced (left bars) and non-induced (right bars) conditions employing pET28a(+)-CelA2, pET28a(+)M1-CelA2, pET22b(+)-BSLA and pET22b(+)M1-BSLA expression systems. Constructs with an M1 label harbor an epMEGAWHOP optimized vector backbone. Enzyme activity levels were determined with the corresponding screening systems in 96-well microtiter plate formats for CelA2 and BLSA. The reported values are the average of three 96-well microtiter plate measurements in which each hydrolase was expressed 8 times per plate and deviations are calculated from the corresponding mean values.