Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Overview of the expression systems used for developing and validating the epMEGAWHOP method
| Expression host | Plasmid | Enzyme | Location of expressed enzyme |
|---|---|---|---|
|
E. coli BL21-Gold (DE3) |
pET28a(+) |
Cellulase (CelA2) |
Intracellular |
|
E. coli BL21-Gold (DE3) |
pET22b(+) |
Lipase A (BSLA) |
Periplasmatic |
| B. subtilis DB104 | pHY300PLK | Protease (subtilisin Carlsberg) | Extracellular |
