Table 2.

Sequencing results of vector backbones that were subjected to epMEGAWHOP optimization

pET28a(+)-CelA2					pET22b(+)-BSLA					pHY-scarlsberg
bp	Substitution			Region	bp	Substitution			Region	bp	Substitution			Region
	wt	-	M1			wt	-	M1			wt	-	M1
488	A	-	T	f1 origin	123	T	-	C	T7 term	209	A	-	G	Tet
850	G	-	C	Kan	2007	A	-	G	na	684	A	-	G	Tet
2233	T	-	C	pBR322 ori	3701	A	-	G	Tet	1584	T	-	C	pTet
2396	T	-	C	na	4563	T	-	C	lacI	3238	T	-	C	pAMP
3170	T	-	C	na	4794	C	-	T	lacI	4221	T	-	C	na
5193	A	-	T	T7 prom						4332	A	-	-	ori-177

The positions of the mutations in the corresponding vector backbones are based on the sequenced vector backbone prior epMEGAWHOP. Alignments of all three vector systems to the corresponding ‘parents’ are included in Additional file 1: Figure S1. Nucleotide position 1 is the base after the stop codon (ATT, TAA) of the inserted genes.

na: no corresponding function of the gene sequence could be assigned.

Kan/Tet: Kanamycin/Tetracyclin resistance gene.

pTET/pAMP: promoter region Tetracyclin/Ampicilin resistance gene (identified with a promoter prediction tool (Reese 2001)).