TABLE 1.
Observational Comparison of Embedding Media Performance for MALDI-MSI
| Embedding media | Physical property | Stability | Ion suppression | Media acceptability |
|---|---|---|---|---|
| OCT | Pliable | Stable | Yes | Poor |
| TBS | Pliable | Stable | Yes | Poor |
| Gelatin (10%, 5%) | Hard | Partial | No | Acceptable |
| Agarose (1%) | Hard | Disintegrated | N.T. | Poor |
| CMC (5%, 2%) | Hard, semihard | Disintegrated | N.T. | Poor |
| Ice (pure MQH2O) | Hard | Disintegrated | N.T. | Poor |
| Sucrose (0.6 M) | Soft | No integrity | N.T. | Poor |
| 2% CMC + 5% gelatin | Semihard | Stable | No | Acceptable |
| 5% CMC + 10% gelatin | Pliable | Stable | No | Optimal |
| 1% Agarose + 5% gelatin | Semihard | Partial | No | Acceptable |
| 0.6 M Sucrose + 5% gelatin | Semihard | Partial | No | Acceptable |
N.T., Medium not tested for ion suppression effects as a result of poor stability and physical properties.