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. 2013 Aug 12;13:383. doi: 10.1186/1471-2407-13-383

Figure 7.

Figure 7

HRG-β1 induces EMT through phospho-Smad2-mediated Snail via PI3k/Akt signaling in SK-BR-3 (a) and MCF7 (b) cells. (a) The cells were pretreated for 1 h with vehicle, 10 μM of LY294002, or PD169316 alone or a combination of LY294002 and PD169316 and then treated with 25 ng/ml of HRG-β1. The expressions of vimentin and fibronectin were identified by western blotting. (b) The cells were treated with 25 ng/ml of HRG-β1 after pretreatment with 10 μM of LY294002 or SB203580 alone or a combination of LY294002 and SB203580. Immunoblots were probed with anti-vimentin and anti-fibronectin antibodies. In all cases, β-actin served as a loading control. Data represent the means ± SD of three independent experiments. *P < 0.05, significant difference.