Table 4.
Oligonucleotides used for strain construction
| Primer | Sequence (5′-3′) a |
|---|---|
| MNN1-5H |
AAGCTTtctacagtggctgttaataatg |
| MNN1-5S |
GCATGCgaaaactcatacaattcactaag |
| MNN1-3A |
GGTACCtgcttactcaagtgttggtg |
| MNN1-3B |
GAGCTCgttagtggcttgtatcaaagg |
| MNN12-5H |
AAGCTTatcataacacgttcgttcagc |
| MNN12-5S |
GCATGCtgacagatcatcgtcttcatc |
| MNN12-3A |
GGTACCatgccattattccaccaatgg |
| MNN12-3B |
GAGCTCgttgtgactgtggctccac |
| MNN13-KO-F |
gttgactttgaagagccactgtatactttccctaagacggtgctatttgaaaacaacaacaatttggatgatttattatGCTCGGATCCACTAGTAACG |
| MNN13-KO-R |
ttaatattttccaacccatacatctcctaagtaattgaaatatgcaatttcttcaggggtatactcaaccaataaaccaCCAGTGTGATGGATATCTGC |
| MNN14-KO-F |
atgcattttatttggcaatcacaagccaattctggattaatactaaaaaatgaattatcactgacatcaagtatcccccGCTCGGATCCACTAGTAACG |
| MNN14-KO-R |
ctataaataattatatcccctcatccatatctccccaagctttttaaaatgttctatttcctctggtgaataattaattCCAGTGTGATGGATATCTGC |
| MNN15-KO-F |
cgtataagggaacttttacatgatagtgaaaaatttgatctcgagagtctaaagcaagctagcttggagataagaaaagGCTCGGATCCACTAGTAACG |
| MNN15-KO-R |
ctttatccgaaccagttccaagtttctcagtttctaatttaacttttggcttcattttggatccaccagtgtgccatatCCAGTGTGATGGATATCTGC |
| MNN16-KO-F |
gaagttccatcttaaaagatatgtcatagttacttcaatactactatcttttttcttattattcagacgacaatttcttGCTCGGATCCACTAGTAACG |
| MNN16-KO-R | ttaatcttgtaaccaaatttcaattaatttcttatattttttttgtaaactttgttcaacattaatagataatcctctaCCAGTGTGATGGATATCTGC |
a Gene-specific sequences are lowercase.