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. 2013 Jul 19;11:33. doi: 10.1186/1477-5956-11-33

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Copyright © 2013 Akada et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Improvement of correlation between dual signals by SDS/2ME/heat pre-treatment. Alexa555 fluorescence is plotted vs. Alexa633 fluorescence for each spot for chips spotted with HSP70C and pre-treated with SDS/2ME/heat (closed circles and bold line) or not pre-treated (open circles and dotted line) before incubating with serum no. 193. Each graph contains sixty spots from 10 chips for four different experiments. R² values were determined by linear regression analysis. Detection of HSP70C was enhanced by pre-treatment with SDS/2ME/heat.