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. Author manuscript; available in PMC: 2013 Aug 23.
Published in final edited form as: J Exp Ther Oncol. 2011;9(2):119–127.

Figure 2. CDDO-Me induces ROS generation in colorectal cells which is blocked by NAC, DPI and rotenone.

Figure 2

Subconfluent HCT 8 and HCT-15 cells were pretreated or not with 5 mM NAC (A) or 10 μM DPI (B) or 200 nM rotenone (C) for 2 h before treating with CDDO-Me (2.5 μM) for 24 h. Cells were then reacted with 5 μM H2DCFDA for 30 min at 37°C. Cells were collected and DCF fluorescence was measured by flow cytometry. Flow cytographs show an increase in the mean fluorescence intensity in both cell lines treated with CDDO-Me, which was blocked by NAC, DPI and rotenone.