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. Author manuscript; available in PMC: 2013 Aug 23.
Published in final edited form as: J Exp Ther Oncol. 2011;9(2):119–127.

Figure 3. NAC prevents induction of apoptosis in colorectal cells.

Figure 3

Figure 3

A. Binding of annexin V-FITC. HCT-15 and HT-29 cells were treated with NAC for 2 h prior to treating with CDDO-Me at concentrations of 2.5 and 5 μM for 24 h. Cells were then reacted with 5 μl of annexin V-FITC + PI for 30 min at room temperature. The percentage of annexin V-FITC positive tumor cells was determined by flow cytometry. B. Cleavage of PARP-1 and procaspases. HCT 8 and HCT-15 cells were treated as described above and cleavage of PARP-1 and procaspases -3, -8 and -9 was analyzed by immunoblotting. Each experiment was repeated twice.