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Combinatorial effect of MSA and MDV3100 on AR transactivation in 22Rv1 cells. (a) Cells transfected with ARE-luciferase construct were treated for 3 hr with 2 μM MSA and/or 5 μM MDV3100 in the absence of DHT, or with 2 μM MSA and/or 1 μM MDV3100 in the presence of 1 nM DHT. (b and c) Cells were treated for 6 hr with 10 μM MDV3100 and/or 4 μM MSA in the absence or presence of 1 nM DHT. *p < 0.05 from vehicle control or DHT control. **p < 0.05 from the MDV3100 group.
