Figure 4. Enhanced production of T-helper 17 cytokines in aortas of mice receiving Il27ra−/− bone marrow.
Percent of CD4 T cells expressing tumor necrosis factor (TNF), interleukin (IL)-17A, and interferon (IFN) γ by intracellular staining in aorta (A, left), spleen (B, left), or paraaortic lymph nodes (paLNs) (C, left) of Ldlr−/− mice transplanted with Il27ra−/− (n=6) or wild-type (wt; n=6) bone marrow fed 16 weeks with Western diet. A–C, right, Representative fluorescence-activated cell sorter plot of CD4+TCRβ+ T cells stained for IL-17A and TNF in aortas (A), spleens (B), and paLNs (C) of Ldlr−/− mice transplanted with Il27ra−/− or wt bone marrow fed 16 weeks with Western diet. Mean±SEM; *P<0.05.