Table 4.

Unique nonsynonymous variants of the DLGAP2 gene identified in ASD patients and controls and their functional predictions

Location			In silico analysis
	Nucleotide position	Variants	PolyPhen-2	SIFT	Autism	Control	rs2906569	rs2301963
exon2	c.44 C > T	S15F	probably damaging	affect protein function	U1974		A/A	C/A
	c.277 C > A	R93S	probably damaging	tolerated	U1843		A/G	C/A
	c.545 G > A	R182Q	probably damaging	tolerated	U173		A/G	C/A
	c.574 G > T	A192S	benign	tolerated	U396		A/A	C/C
	c.797 G > T	V234L	benign	tolerated		ZN4215	G/G	C/C
	c.797 G > T	V234L	benign	tolerated		HN616	A/A	C/A
	c.841 C > G	P281A	probably damaging	tolerated	U323		A/G	C/A
	c.841 C > G	P281A	probably damaging	tolerated	U1519		G/G	A/A
	c.841 C > G	P281A	probably damaging	tolerated	U1988		G/G	C/A
	c.841 C > G	P281A	probably damaging	tolerated		ZN4014	A/G	missing
	c.841 C > G	P281A	probably damaging	tolerated		HN449	A/G	C/A
	c.841 C > G	P281A	probably damaging	tolerated		HN581	A/G	C/A
	c.970 A > T	R324W	probably damaging	affect protein function	U1803		A/A	C/A
exon4	c.1262 C > T	A421V	benign	tolerated		ZN4205	G/G	A/A
exon5	c.1516 T > C	C506R	probably damaging	affect protein function	U1247		missing	C/A
exon9	c.2135 C > T	T712M	benign	tolerated	U2096		G/G	C/A
	c.2135 C > T	T712M	benign	tolerated		HN576	missing	C/A
	c.2135 C > T	T712M	benign	tolerated		HN278	A/A	C/A
	c.2311 C > G	P771A	benign	tolerated		ZN4182	A/A	C/C
	c.2386 C > G	H796D	probably damaging	affect protein function		ZN4053	A/G	C/A
	c.2392 G > C	E798Q	probably damaging	affect protein function	U1082		A/A	C/C
exon11	c.2650 G > A	D884N	benign	tolerated		HN526	G/G	C/A
	c.2676 C > A	N892K	possibly damaging	affect protein function		ZN4107	missing	missing
exon12	c.2750 C > T	P917L	benign	tolerated	U1000		A/G	C/A
	c.2750 C > T	P917L	benign (0.002)	tolerated	U2098		G/G	C/A
	c.2750 C > T	P917L	benign (0.002)	tolerated		HN444	A/A	C/C
PolyPhen-2	autism	control	P (Fisher’s test)	SIFT	autism	control	P (Fisher’s test)
damaging	9	5	0.12	functional	4	2	0.32
benign	4	8		tolerated	9	11