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Sensitivity analysis of nested PCR and ITS1-PCR.L. tropica DNA was diluted 10-fold starting from 103 pg and tested for amplification. The L. tropica nested PCR amplify the 400 bp product (A), and ITS1-PCR amplify about 320 bp product (B). N: Negative control. 100 bp weight molecular (WM) (Bioline).
